fluorescence spectroscopy

Albumin Conformational Changes During Glycation and Thermal Denaturation Processes Revealed by Fluorescence Spectroscopy and Small-angle X-ray Scattering

Authors: Rovnyagina N. R., Tikhonova T. N., Molodenskiy D. S., Shirshin E. А.

Background and Objectives: Objects of the research in this study are solutions of bovine serum albumin (BSA) and its aggregates. Structural changes of the protein molecules in solution with pH 3 and pH 7.4 are investigated during glycation and thermal denaturation processes, when the BSA molecules in solution undergo similar intermediate states. The main aim of the research is to compare structural changes of the BSA upon its glycation and thermal denaturation, revealed by combination of optical and X-ray techniques.

Investigation of the Interaction and Dynamics of Collagen and Collagenase Molecules in Solutions by Dynamic Light Scattering

Authors: Sergeeva I. А., Khitrina K. А., Krot A. R., Sukneva A. V., Petrova G. P.

Background and Objectives: Bacterial collagenase from Closrtidium histolyticum is widely used as a clinical tool in the nonsurgical treatment of Dupuytren’s disease in eye’s disorders treatment, for enzymatic debridement, for accelerated resorption of catgut sutures. Collagenase main feature is its ability to digest key protein of the animal extracellular matrix – collagen. Dynamic Light Scattering (DLS) technique allows for investigation of collagen and collagenase solutions in conditions close to physiological.