interference microscopy

Effect of Spectral Properties of Image Sensor on Interference Experiment

Authors: Kalyanov A. L., Lychagov V. V., Smirnov I. V., Ryabukho V. P.

Influence of spectral properties of monochrome image sensor on spectrum of detecting radiation in fullfield lowcoherence microinterferometer is investigated. Dependence of interference pulse shape and period of interference fringes on the spectrum is shown. Simulated results and experimental measurements of integral transmission spectrum of Linnik microinterferometer with the silicon monochrome image sensor and thermal light source are presented.

Investigation of «Erythrocyte – Plasma» Complex in Native Human Blood Slide Using WhiteLight FullField Microinterferometry

Authors: Kalyanov A. L., Malinova L. I., Bogolyubova E. V., Smirnov I. V., Lychagov V. V., Ryabukho V. P.

Morphofunctional properties of erythrocytes in native blood slide of coronary heart disease patients are investigated using white light fullfield microinterferometry. Three dimensional images and surface profiles of complex «single RBC – surrounding blood plasma» are shown. Dynamic of slope angle of the plasma near the RBC during standardized intravenous glucose load is explored. The dynamic of patients with coronary heart disease is compared with one of patients with coronary heart disease and diabetes mellitus.

Methods of Digital Holography in Interference Microscopy of Reflective Object in Partially Coherent Light

Authors: Savonin S. A., Ryabukho P. V., Lyakin D. V., Ryabukho V. P.

The theoretical foundation of the possibility of using the interference microscopes with extended and frequency broadband light source for recording digital holograms of microscopic objects, reconstruction and visualization of phase images is presented. Numerical procedure for processing of spatially localized digital holograms of the focused image in a partially coherent light are considered. Results of experiments on digital holographic microscopy of biological objects in a partially coherent light with Linnik interference microscope are shown.