Background and Objectives: Traditional methods for measuring the acceleration by changing the position of extremums on the time axis, as well as methods based on the use of least squares and wavelet analysis, require significant signal processing efforts: filtering and allocating extremums or significant time for processing an autodyne signal. The proposed method for measuring the acceleration of the spectrum of the self-mixing signal uses a well-established machine method of Fourier analysis, which is widely used for processing complex waveforms.