Multilayered structures composed by Langmuir – Blodgett films of CdSe/CdS/ZnS quantum dots functionalized by oleic acid and fluorescent at 525 and 631 nm were prepared and studied by fluorescent spectroscopy. It was shown that fluorescent spectra of the nanosized multilayered film consisting of different QD layers have complex character and can be rearranged with relative positions of QD layer and laser beam that open possibility to operate the fluorescent intensity of every QD layer.